使用机械分散机(Dispermat)混合疏水蛋白,SDS和纤维。分散机速度可调,但通常在实验中使用约3800rpm。研究泡沫体积随时间的变化。通过此设置,发泡在1分钟内达到最大水平。通过摄影监测泡沫体积的变化,其中每10秒拍摄照片。从照片图像中,确定达到50%气体体积(t50)和66%气体体积(t66)所需的混合时间。此外,测定达到的最大体积(Omax)。记录10分钟测量泡沫的塌陷率,而每1分钟拍摄照片。根据这些数据,可以估算泡沫的V /。 V /值描述当液体体积达到其初始高度的一半(排水量)时停止混合后经过的时间。
Mechanical mixer (Dispermat) was used to mix hydrophobin, SDS and fibers. Mixer speed was adjustable, but generally about 3800 rpm used in the experiments. Foam volume was studied as a function of time. Foaming reached the maximum level within 1 min with this set-up. The change of foam volume was monitored by photography,where pictures were taken every 10 s. From the photographic images, the mixing times required for reaching 50% gas volume (t50) and 66% gas volume (t66) were determined. In addition, the maximal volume achieved (Omax) was determined.Collapse rate of foam was measured with 10 min recording, whereas pictures were taken every 1 min. From these data V/ for the foam can be estimated. The V/ value describes the time elapsed after cessation of mixing when liquid volume reaches half of its initial height (drainage volume).